Composite

Part:BBa_K3762007:Design

Designed by: Andrea Grimsdatter Stallvik, Martin Eide Lien   Group: iGEM21_NTNU-Trondheim   (2021-09-11)


Reactive sulfur species sensor with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 893
    Illegal NheI site found at 916
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 848
    Illegal XhoI site found at 1192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


Design Notes

This composite part was synthesized by IDT as a linear sequence, and cloned into a plasmid vector using Gibson assembly. The success of the cloning was verified through sequencing.


Source

The genes Psqr, and SqrR were sourced from genome of Rhodobacter Capsulatus SB1003 (GenBank accession no. CP001312), and then codon-optimized using the codon optimization tool from IDT, as described on the individual part pages. The other parts in this composite part are existing biobrick parts from the registry.

References